NurdRage - 2012-10-14
In this video we show how to make fluorescein, a strongly fluorescent chemical. Combine 166 mg of phthalic anhydride, 220mg of resorcinol and 3 drops of concentrated sulfuric acid. Heat to 200 degrees celsius for 5 minutes. The crude fluorescein produced is dissolved in dilute sodium hydroxide solution (300mg in 10mL of water). To demonstrate fluorescence a few drops of the solution are dropped into water under ultraviolet light.
The more I learn at school, the better I understand your videos. I love them.
If contained inside a glass container with a cap, would it be safe enough for an actual lava lamp usage?
Spectacular simple experiment, nice work Nurdrage ! :) I would like to ask if it is possible for You synthesize more chemiluminescent dyes such a Perylene or Rubrene, I synthesized Perylene once and reaction beetwen MNPO and Perylene was very cool.
It looks lovely !
You need the sulfuric acid to make the reaction happen, H2SO4 is needed to take water out of the way and drive the reaction. You put sodium hydroxide in anyway, which would neutralise the acid as well as help dissolve.
Great video. That's really cool.
this would make great animated screen-saver too!
Hey, can u tell me if Fluorescin is a good substance to use to track water movement?? would like to detect the differences in laminar and turbulent water movement, don't want to dilute in water in order to see the change in movement
Can I use this as a dye in the peroxy-oxalate chemiluminescene reaction (the TCPO glow-stick reaction)? Could someone please help me. I need this information for my school project.
This really makes me picture a creature messing with people's souls in a container. It looks so amazing. Thank you for the video. Science is awesome.
your videos own !! keep it up !! :)
could you make a video (pro video) how to make a lavalamp?
It actually isn't. I've performed this exact experiment as my ap chem demo and it worked exactly as shown.
beautiful
Can you use Fluorescein as the dye in the glowstick?
WICKED!!! =D AMAZING!!
sweet :D , will be waiting for that one
Is there any way to form a salt from this?
Could fluorescein be used as a dye in the video where you made different colored glow stick reactions?
"Greetings fellow nerds" DAMN RIGHT
Please i am looking for the most cost effective light that will cause di-sodium fluorescein , acridine orange and rhodium b to react. I have been growing map crystals and want to see how well these substances will work, but there are so many types of lights and many are expensive while those that are cheap look terrible. From what I understood uv light is invisible so the cheap lights throwing out a ton of bright light causes hesitation. please help.
i'll be uploading a new video on fake chemical blood in an hour or so.
Really cool, I love all your videos good sir! You make me want to be a chemist!
@NurdRage out of the approx 10ml of concentrate you made how many litres of water can you make luminescent. Would adding bleach destroy the fluorescence effect? Im thinking about mixing this with 0.05% bleach solution to allow for spray disinfection ofhard surfaces with a blacklight verification that all surfaces are adequately decontaminated. Could that work? Also is fluorescine toxicity at factor?
Woo!!!! new video!! I live for these.
God i love your videos lol
I've been trying to figure out the mechanism for this reaction but just can't seem to do it. I can push arrows all the way through to have all 3 components condensed together, but I just can't seem to go on. I mean I managed to create a Michael acceptor through a lot of protonation/eliminations, but when I close the ring through a conjugate addition, I'm missing one double bond in the final product. :/
just mix ammonium nitrate + diesel 95:5.
I want to try this one, but I've no idea where to find the ingredients..
can i put this on a t-shirt without damaging it or myself?
Would this solution be safe to store in a glass bottle for long periods of time? As a display piece?
It's safe, but the water and fluorescein would combine rather quickly, so there isn't really any point to do that...
Thanks For this video .. Its solved my big problem
make some anfo, and do the chemical formula for that!
My fluorescein purity didn't end up too well :C ... Still ended up being very beautiful though.
If I wanted to dye fabric with fluorescein, do i just dilute in water? Do I need any other ingredients for fabrid dye?
What would happen if you did the glow stick experiment using Fluorescein as a dye, would it glow or would it only work under UV light?
What if his voice is just naturally that deep..
You're good at words.
the new and inproved lava lamp!
Neat!
While I'm sure you're right about small scale production I've often wondered about the large scale production that takes place. I'm a trained organic chemist with years of bench time and I would need to train up to use industrial scale equipment and processes as you simply don't come across it doing normal lab chemistry. My conclusion is that some of the drug production must be done by highly trained individuals
Sulphuric acid isn't totally necessary to catalyze the reaction, the reaction mechanism consists of going through a series of protonation/deprotonation and carbonyl attacks, so really any proton source would be fine. Any acid proton source. I'd guess his acid is probably somewhere around the 2-5M concentration given how viscous and yellow it was.
Does anybody know where I can get these kinds of chemicals? Thanks
How do you do your experiments with your camera? Do you wear a gas mask usually so you can work freely without the fume hood? Also do any of the chemical reactions harm your camera?
Very cool!
Very cool!
See his video on how to make luminol the full guide. He shows how to make phthalic anhydride. On the other hand, I don't know where to get resorcinol.
I bought some of this in a powder on ebay about 10 years ago for dirt cheap. It was a blast.
Mate, where do you get all your reactives?
ino ,oj - 2012-10-15
Beautiful video as always...this is the best art