> chemistry > techniques-et-matériel > making-thin-layer-chromatography-tlc-plates-thehomescientist

The Home Scientist 024 - Making thin-layer chromatography (TLC) plates

TheHomeScientist - 2010-05-23

How to make thin-layer chromatography plates (TLC plates) for a few cents each that are as good as commercial TLC plates that sell for a dollar or two each. You can use these home-made TLC plates the same way you'd use chromatography paper, but the plates provide sharper separations and require far less analyte. They also lie flat, and are much easier to store for later reference.

BrotherBloat - 2010-05-23

rich content as always - thank you!

Kevin L - 2015-12-15

Thank you for this.

Bryan Elliott - 2010-05-24

Pretty awesome info for the mad scientist on a budget ^_^

francis lavoie - 2010-05-23

pretty interesting

zcuttlefish - 2010-05-29

Hey RBT thanks for putting this video up! I use this everyday in lab (along with flash chromatography). I had been wondering what the binding agent was (I would think that the solvents would dissolve the binder). I think it would be cool if you talked a little about the principle of TLC bc most people won't have the solvents, uv lamp, or stains necessary. Cheers!

Joy Baker - 2016-10-28

Thanks! Great video. So helpful for teachers without much budget!!!

Sully Science - 2010-05-24

I'm working on my supply orders for school next year and you just saved me a lot of money. Thanks for the great information.

nehorlavazapalka - 2010-05-29

Fail, I've accidentaly arrived at this video while having the TLC plate in front of me.

nick paz - 2010-05-23

nice video!

olympicfan2 - 2010-05-24

very good!

AoiHana - 2015-08-11

good blese

Spencer Burrows - 2014-06-16

what are the amounts again?

Soraslight - 2010-05-23


searodrig - 2013-11-27

do you have a website where these instructions are listed? That would be awesome!

DutchPhlogiston - 2013-08-04

Interesting. I wander how well your alumina/plaster mix would perform in a homemade HPLC-like column...

XN283298 - 2016-12-24

if so made plate will glow in UV ?

Spackolatious - 2017-12-12

A slide prepared this way will not fluoresce or glow unter UV irradiation by itself (unless your substance is a fluorophore itself), for that you will need a Mn-doped Zn2SiO4 layer which will absorb UV light at 254 nm

G Money - 2010-05-23


Karl Thorne - 2010-05-25

looks good, hey if it costs less to make them, make them yourself and sell them online for just under the store price and make a small buck =]

gglafatottari Drullusukkor - 2012-06-15

1.25ml per gram alumina and 15% of binder ???????????? so 1.25 grams of water to 1 gram of alumina to 0.3375 grams of binder or 1.25 grams of water to 1 gram of alumina to 0.15 grams of binder

Josh Ginges - 2011-10-16

How much does the binder affect retention? Is it possible to use this technique, using the same binder, with polymer-based reversed-phase media? Thanks for the great demo!

krystal Teque - 2013-04-01

what would u use for colouring as most tlc use fluorescent??

Kristoff B - 2010-12-14

It's worth adding that previously reagents like Dragendorff or ninhydrin mentioned in your previous videos work beautifuly with TLC, giving an extra dimension for identification of substances. And even without reagents you can make nice separations eg. dyes and inks (which is used in forensic investigations of documents when there's a suspicion of forgery).

Rust Lord - 2016-01-28

Excellent video.

Here now - 2021-02-11

Very succinct I like it.

skagsux - 2013-07-25

Is it possible to use powdered granules from silica gel packs that come in a vitamin bottle or with your new pair of shoes (you know these desiccant packs). Also is it possible to use the immobilized phase you detail with highly hydrophobic (oily, resinous etc) compounds.