Thy Labs - 2023-04-04
In todays video I will try to extract the deadly tropane alkaloids from datura stramonium. This plant contains atropine, scopolamine and hyoscyamine. As all of these alkaloids are deadly you should not repeat this. We might have failed to obtain pure crystals of said alkaloids but the finished extract is extremely toxic anyways. Get a 7% discount from laboratoriumdiscounter.nl by using the 7% discount code "THYLABS2023". They sell most of the things you will ever need as a chemist and much more. Support me on patreon for even crazier experiments: Patreon: https://www.patreon.com/thyzoid Discord: https://discord.gg/UrjAt44HWH Instagram: https://www.instagram.com/thyzoyd Twitter: https://twitter.com/thyzoid1 TikTok: https://www.tiktok.com/@thylabs Reddit: https://www.reddit.com/r/THYZOID/ It's been a long journey on YouTube but I will not continue with this forever. The likelihood that I will stop on 14th of April 2025 is very high so enjoy (and archive) the videos as long as I am producing them. It is not a wise choice for me to continue for longer. I just don´t care anymore. At least It gave me and the time put into this some form of meaning.
We will try this again. I want this to work out perfectly. Error in the video: The tropic acid video is still being edited. It will be uploaded in less than a month.
I posted a procedure that works great for extraction from seeds. The oils and fats need to be removed first, then you pull the alkaloids next
Hey, it's me from reddit who said I wish to see this video when it's released, I love it, this is awesome shiii booiiiii
@@perfid-deject2027 i´m happy that you enjoyed it
@@THYZOID yep, the next extraction will be bettah
Where did you buy the seeds?
I don't think you would have destroyed the alkaloids, the fluffy flocculant in the ether looked great. Try allowing the particles to aggregated a little and maybe cool the ether solution down to kick out as much of the compounds as possible and then filter through a coffee filter when the particle size has increased a little. You definitely need to do either a filtration or a centrifugation step here to separate all the other junk that has stayed dissolved in the ether, you wont be able to just boil it off.
Please be careful if you do decide to centrifuge stuff in ether. It will explode if it sparks and a spark-proof centrifuge should be used.
It makes sense that it would be very difficult to alter the alkloids but why take any chances by significant loss of yield.
fluffy flocculant ❤
The HCl salt in ether just needed filtering. Heating it probably was a mistake, tropanes can get tricky like that. Cooling the mix in the freezer and filtering would been ideal, then you just dry it at room temp. The fluffy suspension is sexy af by the way.
Or maybe extracting the HCl salt into water from the ether suspension and then evaporating the water or freebasing from there.
I did my college project with one of your videos. I follow you since
Yup that Is a variation on a soxlet extractor. Try brewing coffee in one. It will be the best cup you ever had.❤
Cool video. I extracted nicotine, mo hung in different solvents. I have used isopropyl alc and pyrrolodine to extract stychnine and brucine. For safety I only extracted 1.8 g of nux vomica. That 20 to 35 mg of Strychnine and 28 mg brucine. Sodium dichromate and hcl turned the solution is dark purple.
Where did you get your hands on Mo Hung? Ephedra grows everywhere here in desert mountains but it's not mo hung
First step grind up and dry absolutely in z sealed container that passes air over dry mgso4. 2nd step dry your hexzne, etc, and make it acidic by bubbling HCL gas through it. Then, load into your soxlet extractor. Add this into your reveiver and just enough to soak the seed powder. The alkaloids will absorb some hcl the rest will off gas. Let it run 4 to 6 hours at aggressive reflux. This removes oils and fats. The hexane wash can be discarded. Next, add a bit of Ca(OH)2 on top of the seed mash, and repeat the procedure with ammonia saturated DCM to get alkoids as a free base. Distill off the DCM Clean up can be done by a simple acid base extraction, aim for PH of 2 to 3 for the acid step and 12 to 13 for the base step. Diethyl ether is great for the organic pulling in this step. Weigh the flask so you can calculate the amount of alkaloids present. Add the eter solution of alkaloids and distill off ether weigh again to get your alklod amount and dissolve in anhydrous isopropyl alcohol and make a solution of sulfuric acid in isopropyl alcohol in slightly more than equimolar amount and mix. Your alkaloids will crash out.❤
if you wanna know how bad datura is, look up trip reports. not only is it deadly but it causes delirium/hallucinations.
Yeah like 98% of them trip reports end up in a hospital visit ... My friend once took it too, he was kinda lucky as never badly physically hurt himself, but I'm pretty sure suffered some major mental trauma from it for several years following his "experiment". Truly scary stuff.
@instructoruldemeditatie Yeah agreed, as with anything it's the dose that makes the poison. I'd personally never take this for "fun" though at any dose, as it's not that kind of experience...
@instructoruldemeditatie "i don't know why people want to use substances that trigger psychosis" I think in a lot of cases it's because they are already psychotic to start with. True drug-dustbins don't seem to have much in the way of a self-preservation instinct. The one that always baffles me is the people who take Aminita mushrooms. Why in the name of Sooty's nanny would anybody poison themselves with that crap when Cubensis is safe(ish) and relatively easy to grow?
@@AnthonyHandcockcause amanita works different. Ibotenic acid isnt that bad ?
I've done brugmansia in a controlled setting. It was a hauntingly beautiful experience. Losing the ability to speak or put objects down reveals you for who you truly are deep down on the inside. Waking up to people who love me around me taught me that I had nothing to be afraid of in this world and that without recognition and validation I would be an empty observer. After that experience, I started living life on full blast, I lost my fear of the darkness, I got into graduate school. I think it's important people have bad trips. If you can prepare to control yourself in them, you can control yourself anywhere.
I have experience with alkaloid isolation. Seeds contains huge amount of fat so you should did extraction with pure solvent (hexane or heptane without amonia) first to get rid of fat. It easier to use leafs (without fats). Tropane alkaloids are easy extractable and there is ni need for Soxhlet (these alkaloids are esters so there is some decomposition with hot amonia). Extraction with warm acid water is enough. After that add ammonia and extract into organic solvent.
Yup. Years ago I tried extracting what I thought it penicillin from some moldy coconuts using DCM, but instead of getting a product I ended up with a weird ugly butter, and had no way of removing the fat, so had to destroy my product, since it was useless.
What about acetone? Acetone dissolves fats
The extractor is a continuous liquid/liquid extractor. It is intended to extract things from light liquids (hexanes are common) into water but can also be used to extract things from water into chloroform or other heavy liquid.
It can extract things into a liquid with a poor or even inverse partition coefficient. Ie. Equally or more soluble in the lighter liquid.
sounds reasonable. thanks!
Ricin next! Ricin next! Ricin next!
I sometimes work with these Alkaloids at work, however only in mg/mL or even mg/L concentrations
3:41 Very important step. I was lazy and where slightly high from the potent grease for weeks on end, because it sticks everywhere and spreads everywhere🤣
Aww, im your oldest Patreon <3.
Im glad you created your Patreon. I knew you would get a ton of supporters in very short time. There aren't many chem channels this awesome on YT right now.
Definitely one of my favorites
That cappuccino at 6:55 looks delicious! Enjoy.
wish i had a large industrial sized sohxlet extractor
Thanks 👍
waiting for the second try that was amaizing
You'd better to use acidic water extraction, then basify the solution and do organic solvent extraction
Would this method work with other plant alkaloids? Any information would be greatly appreciated
Anyone else ever wondered where the hell he buys poisonous seeds and chemical glassware and chemicals without being on a watchlist of some kind
I am the watchlist
In Germany you can even ger Benzaldehyde, nitroethane and even p2np as a hobby chemist. Feel bad for hobby chemist who live in a country where even glass Equipment is ilegal. What a shame
And what can you make with those? I'm sure as in many places (like schools) glasswares etc are used so u can't make that illegal.@@wn1757
Who says he's not on a watch list 😂
Ergot alkaloids pls
THY: "basically enslaved death..."
Death: "I BEG YOUR PARDON?"
It baffles me that legally this is perfectly fine but if I wanna extract lsd precursor it's a federal crime
Don’t forget that if they want to sell something with codeine over the counter they purposefully poison it so people don’t get high off it.
Or when they purposefully poison ethanol in hand sanitizers for no reason other than to poison it off people who want to drink it.
Tips for less impurities: acidify the product and wash with nonpolar. then freebase product, wash with water.
When removing solvent, avoid heat. maybe try reduced pressure.
Is there much fat in the seeds? You might want to de-fat the seed powder (without ammonia treatment) with hexane first to remove fats.
Very amazing project! Question! Is there another method, without hexane ? Can I use acetone or any OTC ?
You can show us how to use soxhlet extraction in this extraction
Use diethyl or even subcritical cold methyl ether as a solvent and then tartaric acid to salt when dealing with delicate alkaloids. HCl, H2SO4 and Heptane and Dichloronethabe are too harsh. You could try guanidine as the alkali to freebase, it's milder than Ammonia thats why its used in mild afro hair relaxers.
Chances that an akaloid dissolves in hexane are lousy. I would powderize the seeds and then do test extractions with THF or Ethylacetate or CHCl2 on just a gram of the seeds. Then run a TLC to check if the extraction works. In the first place read up on the available literature!
tried these once, not my brightest idea haha
isnt scopolamine used as a precursor in a lot of medicines? pretty cool stuff!
it is used in medicine. as a precursor and directly as medication for sea sickness
Scopolamine has been known to be used to 'knock out' unsuspecting persons so they can be robbed. I believe I first read of this being used in Colombia.
How many unsuspecting persons *didn't wake up, was never reported, and possibly just an unknown overdose or 'natural' death.
Study shows drastic improvement in people with mood swings dont know what dose😅
can you do it for ricin ? Thank you very much
no
@@THYZOID why not
looool the miniuature : ho that guy is extracting scopolamine from datura? please do a trip repport too ^^
i won´t eat this so no trip report lol
@@THYZOID i understand you about not trying pure scopolamine xD
@@sunoncream1118 Eating Datura it's self is bad enough lol... Would not recommend! xD
Can... can we do that one alkaloid in ergot?
nope. the conditions of this extraction would likely be way to aggressive which would destroy the target compound
@@THYZOID so change the conditions. For science
I‘m pretty sure the fluorescent component is chlorogenic acid, a very common secondary plant metabolite.
Can strongly recommend looking at your first liquid extraction step
yes. next time the seeds will be ground up even more, the alkaloids will be freebased by letting the seeds sit in NH3 for a day before drying them and then doing the usual hexane extraction
I'll just take out seeds from datura plants growing everywhere in our city.Literally thousands of plants.
Will this work to get painkillers out of opium how do you distil heroin & codeine & morphine & thebaines For educational purposes only just in case the world ends and we need to know for survival
Use the plant roots that is where most of the alkaloid production is expressed in that plant.
That's pure madness extract
Heard stories about scapolamine crimes in Colombia. Scapolamine is blown onto a tourist to inhale to make them suggestible, then somebody leads them to the cashpoint so they can willingly give all their money away 😅😬
Yeah it's a dissociative, hypnotic, deliriant. Really dangerous stuff if not handled with care, and almost always involves a visit to the hospital, even when intentionally ingested!!
Yeah usually with Rohypnol thrown in...
Out of curiosity, does the plant product have to be dry to extract the alkaloid properly?
Say, could you extract solanine from green potato plant, do you have to dry the entire stem and then extract the alkaloid, or does it work if you use the green plant?
It is not necessary but preferable.
Good videos!
Fbi agent i swear my youtube was on autoplay!
The crazy thing is Datura and all the compounds it contains are legal in most places, because it's so unpleasant (and literally poisonous), only a handful of brave souls ever try it (I'm intentionally picking a kind adjective, when I can think of many other choice descriptors to attribute to someone who chooses to try Datura willingly lol).
Your "soxhlet-like" apparatus makes me think of an oil/water separator used in the steam distillation of essential oils. I don't think I can share a link in a comment, I'll try right under this one
Can you explain how this is different from a soxhlet? It looks the same (to me), he has a condenser on top, though with a smaller diameter adapter than others that I can recall.
@@z2kk in a soxhlet extractor, the tube on the side is used as a siphon: the main chamber with the things to extract fills up gradually because of the condensation of the vapours of the solvant, and when it's filled enough the whole chamber is emptied of its solvant: that's one cycle.
Here, the tube that could allow the cycling is pressure equalised to the rest of the apparatus, meaning that it can't cycle: it works continuously
@@bilaljanati6214 Ah got it, thx for taking the time.
It is a continuous liquid/liquid extractor like oil water. I put a detailed explanation in its own thread.
Nice ad choice! 😜
I'm wondering what is the benefit of doing this? Just for fun or education? Or could you extract the poison and do the substance without fear of death? I ate some of these seeds on time. Almost killed me. Dumbest thing I ever did
Can you do one extracting LSA from hawaiian baby woodrose seeds or morning glory seeds?
na. illegal here as lsa is covered by the new psychoactive substances act
@@THYZOID Those pesky regulators.
Try Erowid Vaults
@TheTransporter007 - 2023-04-04
Welcome, fellow watchlist members.
@JackFrawley101 - 2023-04-12
When I was a kid I was on the naughty list, now I'm on all sorts of them
@idtyu - 2023-07-02
There's no going back after watching Nile red video about that chemical that people put on rag and make you smell it
@anon-means-anon - 2023-07-04
At this point I don't trust people that aren't on a watch list.
@davidaldrich5941 - 2024-07-07
Is it even possible not to b on these lists .. I feel like it's just the same bluff as our "per minute record
@ingweking8748 - 2025-06-13
Hi